• Home
  • About
  • Services
  • Resources
  • Contact Us
November 15, 2018

Beckman Coulter CytoFLEX LX has been added to our family of Cytometers.

The CytoFLEX LX is equipped with 5 lasers and has the ability of having a 6th one added. The cytometer is able to read 96 well plates and is being used for large multicolor experiments. Training for individuals on this instrument is available. It is an automated walk system when using the plate reader.

Cytoflex 5 laser configuration

More Information

April 5, 2017

BenchSci: Explore published figures to find antibody usage data

Benchsci

BenchSci collected 400,000 antibody validations from 4,000,000 open access papers and are adding 100,000 new validations every month.

April 17, 2014

Training Documents and Presentation

Pre-workshop materials for first-time users

Attune NxT Acoustic Focusing Cytometer User Guide

Attune on site training slides

Compensation_Workshop

April 17, 2014

Useful Resources

blog-post-featured-image

Below is a list of recommended technical resources for flow cytometry

  • Rockerfeller University Flow Cytometry Tips and Resources
  • abcam protocols and troubleshooting tips
  • UC San Diego Flow cytometry Protocols
  • Current Protocols in Flow Cytometry
April 17, 2014

Sample Guidelines for the Cell Sorting

The following sample guideline for cell sorting is adapted from the Rockfeller University. For source, please visit: The flow cytometry resources at Rockfeller University

Sample Guidelines for the Cell Sorting

  • Human samples must be serology tested before brought to the FCRC
  • Samples suppose to be single cell suspension (SCS) only (i.e. no clumps). Use all applicable Sample Preparation Tips
  • If you have extensive cell death in the sample, add DNAse (see Sample Preparation Tips). It will not only decrease clump formation in the sample, but also will decrease viscosity of the single cell suspension buffer, by digestion of the free DNA. Sort purity and yield could go substantially up after DNAase treatment
  • Add the 10-25mM of HEPES, pH range 6.8 – 8.2 (for example: HEPES buffer solution 1M in H2O, Sigma-Aldrich Co., Cat# 83264-100ML-F) to your buffer. Addition of HEPES will significantly increase the buffer capacity of the original sample buffer. Buffer capacity of the common phosphate and carbonate buffers gets compromised by high pressure within the instrument during the cell sorting procedure

Continue reading →

Recent Posts

  • Second Attune NxT
  • Beckman Coulter CytoFLEX LX has been added to our family of Cytometers.
  • BenchSci: Explore published figures to find antibody usage data
  • Training Documents and Presentation
  • Useful Resources

Categories

  • News
  • Protocols
  • Resources
  • Training
    • Core Services
      • Centre for Advanced Technologies
      • Centre for Mouse Genomics
      • Experimental Imaging Centre
      • Microscopy and Imaging Facility
      • Southern Alberta Mass Spectrometry
      • Core DNA Services
    • Featured Sections
      • Contact Us
      • Services
      • Facility Personnel & Instruments

Find Us

The flow cytometry facility is located in the basement of Health Sciences Centre (HSC) room B016 at the Foothill Campus of the University of Calgary.

© 2017 | All rights reserved

Flow Cytometry | University of Calgary

  • Home
  • About
  • Services
  • Resources
  • Contact Us