The CytoFLEX LX is equipped with 5 lasers and has the ability of having a 6th one added. The cytometer is able to read 96 well plates and is being used for large multicolor experiments. Training for individuals on this instrument is available. It is an automated walk system when using the plate reader.
Below is a list of recommended technical resources for flow cytometry
The following sample guideline for cell sorting is adapted from the Rockfeller University. For source, please visit: The flow cytometry resources at Rockfeller University
Sample Guidelines for the Cell Sorting
- Human samples must be serology tested before brought to the FCRC
- Samples suppose to be single cell suspension (SCS) only (i.e. no clumps). Use all applicable Sample Preparation Tips
- If you have extensive cell death in the sample, add DNAse (see Sample Preparation Tips). It will not only decrease clump formation in the sample, but also will decrease viscosity of the single cell suspension buffer, by digestion of the free DNA. Sort purity and yield could go substantially up after DNAase treatment
- Add the 10-25mM of HEPES, pH range 6.8 – 8.2 (for example: HEPES buffer solution 1M in H2O, Sigma-Aldrich Co., Cat# 83264-100ML-F) to your buffer. Addition of HEPES will significantly increase the buffer capacity of the original sample buffer. Buffer capacity of the common phosphate and carbonate buffers gets compromised by high pressure within the instrument during the cell sorting procedure