The following sample guideline for cell sorting is adapted from the Rockfeller University. For source, please visit: The flow cytometry resources at Rockfeller University

Sample Guidelines for the Cell Sorting

• Human samples must be serology tested before brought to the FCRC
• Samples suppose to be single cell suspension (SCS) only (i.e. no clumps). Use all applicable Sample Preparation Tips
• If you have extensive cell death in the sample, add DNAse (see Sample Preparation Tips). It will not only decrease clump formation in the sample, but also will decrease viscosity of the single cell suspension buffer, by digestion of the free DNA. Sort purity and yield could go substantially up after DNAase treatment
• Add the 10-25mM of HEPES, pH range 6.8 – 8.2 (for example: HEPES buffer solution 1M in H₂O, Sigma-Aldrich Co., Cat# 83264-100ML-F) to your buffer. Addition of HEPES will significantly increase the buffer capacity of the original sample buffer. Buffer capacity of the common phosphate and carbonate buffers gets compromised by high pressure within the instrument during the cell sorting procedure

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